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Invitrogen™ eBioscience™ Mouse MIP-1 alpha (CCL3) ELISA Ready-SET-Go!™ Kit
Specifically engineered for accurate and precise measurement of mouse MIP-1 alpha protein levels from samples including serum, plasma and cell culture supernatants. MIP-1 alpha and MIP-1 beta belong to the family of cysteine-cysteine (cc) chemokines.
Manufacturer: Invitrogen™ LS885601322
Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Mouse MIP-1 alpha Standard (lyophilized): 100pg/mL upon reconstitution
Coating Buffer Concentrate
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution
96 Well Plate included with product Cat. #'s ending in suffixes -22, -76, -86
Description
CCL3 (MIP1a, Macrophage Inflammatory Protein-1 alpha, MIP-1 alpha) has a molecular weight of approximately 8 kDa, belongs to the family of chemotactic cytokines, and is involved in chemotactic and proinflammatory effects, and homeostasis. CCL3 is produced by lymphocytes, macrophages and dendritic cells, and is involved in inflammatory response of blood monocytes and tissue macrophages. Also, CCL3 can exist as a naturally occurring heterodimer with MIP-1beta and has been shown to have antiviral activity against HSV-1. CCL3 protein can bind with high affinity to the CCR1 and CCR5 receptors.Specifications
| P10855 | |
| Biotin | |
| 20302 | |
| Colorimetric Microplate Reader | |
| ELISA | |
| RUO | |
| Plasma, Serum, Supernatants | |
| Plasma (50μL), Serum (50μL), Supernatants (50μL) | |
| 2-8°C | |
| 24 hr 30 min | |
| Ccl3 | |
| HRP |
| 0.8-50 pg/mL | |
| ELISA | |
| 96-well plates | |
| Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) | |
| 2 x 96 tests | |
| 0.8 pg/mL | |
| 50μL | |
| Mouse | |
| 1 hr 45 min | |
| Mouse | |
| Small inducible cytokine A3, SCYA3, CCL3 |