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  5. Invitrogen™ Mouse IL-9 Uncoated ELISA Kit

Invitrogen™ Mouse IL-9 Uncoated ELISA Kit

Catalog No. 501125217
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10 x 96 Tests
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501125217 10 x 96 Tests
501125215 20 x 96 Tests
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Catalog No. 501125217 Supplier Invitrogen™ Supplier No. 88809288
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Description

ELISA

The Mouse Interleukin 9 (IL-9) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-9. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

Accession Number P15247
Assay Range 32-4000 pg/mL
Assay Sensitivity 32 pg/mL
Content And Storage Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.
96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22, -76, and -86)
Conjugate HRP
Immunoassay Kit Format Uncoated ELISA Kit
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40; IL-2
Gene ID (Entrez) 16198
Gene Symbol Il9
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye Biotin
Protein Aliases IL-9, Cytokine P40, T-cell growth factor P40
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 100 μL, Supernatant 100 μL
Storage Requirements 2-8°C
Synonym Interleukin-9, T-cell growth factor P40
Target IL-9
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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