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PeproTech Mouse IL-10 ELISA Development Kit (TMB), PeproTech®

Catalog No. 900T53K
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10 x 96 Tests
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900T53K 10 x 96 Tests
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Catalog No. 900T53K Supplier PeproTech Supplier No. 900T53K

Description

ELISA

900-T53K consists of: 900-T53 (capture antibody, detection antibody, and protein standard), and HRP-17UL (streptavidin HRP-conjugate). Mouse IL-10 TMB ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinantmouse IL-10 in a sandwich ELISA format. Mouse IL-10 TMB ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells. The TMB ELISA Buffer Kit (Product # 900-T00), sold separately, is recommended to be used in conjunction with this TMB ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

Accession Number P18893
Assay Range Lot-specific
Assay Sensitivity Lot-specific
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Growth Factors & Receptors
Target Species Mouse
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF;
Gene ID (Entrez) 16153
Gene Symbol IL10
Kit Contents This Mouse IL-10 ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately., Detection Antibody, Protein Standard, HRP-conjugate
Label or Dye Biotin
Quantity 10 x 96 Tests
Regulatory Status RUO
Shipping Condition Room temperature
Storage Requirements Refer to product documentation for component specific storage temperature.
Target IL-10
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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