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Invitrogen™ eBioscience™ Mouse Ig Isotyping ELISA Ready-Set-Go!™ Kit

Specifically engineered for measurement of mouse Immunoglobulins from cell culture supernatants. Up to 12 individual samples can be tested for anti-IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM as well as light chain kappa and lambda.

Manufacturer:  Invitrogen™ LS885063086

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Catalog No. 501125158



Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Mouse Ig isotype control mixture (positive control), lyophilized
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)

Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

HRP
96-well plates
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
10 x 96 tests
Supernatants
Supernatants (400μL)
2-8°C
24 hr 30 min
HRP
ELISA
Colorimetric Microplate Reader
ELISA
RUO
50μL
Mouse
1 hr 45 min
Mouse
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