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Invitrogen™ Mouse HO-1 ELISA Kit

Catalog No. EEL141
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96 Tests
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Catalog No. Quantity
EEL141 96 Tests
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Catalog No. EEL141 Supplier Invitrogen™ Supplier No. EEL141
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ELISA

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

Specifications

Accession Number P14901
Assay Range 62.5 to 4000 pg/mL
Assay Sensitivity 37.5 pg/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 15368
Gene Symbol HMOX1
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target HO-1
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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