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Invitrogen™ Mouse Creatine Kinase MM ELISA Kit

Catalog No. EEL217
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96 Tests
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EEL217 96 Tests
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Catalog No. EEL217 Supplier Invitrogen™ Supplier No. EEL217

Description

ELISA

Mouse Creatine Kinase MM ELISA Kit quantifies Creatine Kinase MM in serum and plasma. The Mouse Creatine Kinase MM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Specifications

Accession Number P07310
Assay Range 1.56-100 ng/mL
Assay Sensitivity 0.94 ng/mL
Conjugate HRP
Detection Method Colorimetric
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum
Target Species Mouse
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias CKM; CKMM; CPK-M; Creatine kinase M chain; creatine kinase M-type; creatine kinase, M-type; creatine kinase, muscle; creatine phosphokinase M-type; M creatine phosphokinase; MCK; M-CK; muscle creatine kinaseCKM; CKMM; CPK-M; Creatine kinase M chain; creatine kinase M-type; creatine kinase, M-type; creatine kinase, muscle; creatine phosphokinase M-type; M creatine phosphokinase; MCK; M-CK; muscle creatine kinase;
Gene ID (Entrez) 12715
Gene Symbol CKM
Interassay CV <10%
Intraassay CV <10%
Kit Contents Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma: 100 μL, Serum: 100 μL
Shipping Condition Wet Ice
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Creatine Kinase MM
Test Time 1 hr 20 min
Total Assay Time 3 hr 30 min
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