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Invitrogen™ Monkey Transthyretin ELISA Kit

Catalog No. EEL215
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96 Tests
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Catalog No. Quantity
EEL215 96 Tests
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Catalog No. EEL215 Supplier Invitrogen™ Supplier No. EEL215

Description

ELISA

Monkey Transthyretin ELISA Kit quantifies Transthyretin in serum and plasma. The Monkey Transthyretin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Transthyretin (TTR), generally called prealbumin, is a plasma protein that plays an important role in physiology such as a transporter of hormone thyroxine and retinal-binding protein. After produced primarily in the liver, TTR is excreted into the plasma. TTR represents a disproportionate fraction (25%) of CSF protein, prompting the suggestion that it is either selectively transported across the blood-CSF barrier or synthesized de novo within the central nervous system. Transthyretin is a constituent found to the neuritic plaques, neurofibrillary tangles, and microangiopathic lesions of senile cerebral amyloid. It has been reported that more than 40 different mutations in the TTR gene associated with amyloid deposition.

Specifications

Assay Range 0.31-20 μg/mL
Assay Sensitivity 0.19 μg/mL
Conjugate HRP
Detection Method Colorimetric
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum
Target Species Monkey
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias AA408768; AI787086; Amyloidosis I; ATTR; carpal tunnel syndrome 1; CTS; CTS1; D17860; epididymis luminal protein 111; HEL111; HsT2651; Lr1; PALB; prealbumin; pre-albumin; prealbumin, amyloidosis type I; Tbpa; thyroxine-binding prealbumin; transthyretin; Tt; TTRAA408768; AI787086; Amyloidosis I; ATTR; carpal tunnel syndrome 1; CTS; CTS1; D17860; epididymis luminal protein 111; HEL111; HsT2651; Lr1; PALB; prealbumin; pre-albumin; prealbumin, amyloidosis type I; Tbpa; thyroxine-binding prealbumin; transthyretin; Tt; TTR;
Interassay CV <10%
Intraassay CV <10%
Kit Contents Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma: 100 μL, Serum: 100 μL
Shipping Condition Wet Ice
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Transthyretin
Test Time 1 hr 20 min
Total Assay Time 3 hr 30 min
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