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  5. Invitrogen™ Monkey Osteoactivin/GPNMB ELISA Kit

Invitrogen™ Monkey Osteoactivin/GPNMB ELISA Kit

Catalog No. EP24RBX5
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5 x 96 Tests
96 Tests
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EP24RBX5 5 x 96 Tests
EP24RB 96 Tests
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Catalog No. EP24RBX5 Supplier Invitrogen™ Supplier No. EP24RBX5

Description

ELISA

Monkey Osteoactivin/GPNMB quantitates monkey Osteoactivin/GPNMB in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant monkey Osteoactivin/GPNMB.

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

Accession Number Q14956
Assay Range 0.12-25 ng/mL
Assay Sensitivity 0.12 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human, Monkey
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Dchil; DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; glycoprotein nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; Gpnmb; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; Hgfin; ipd; iris pigment dispersion; NMB; Osteoactivin; si:ch211-155k24.6; transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB; Unknown (protein for MGC:133733); UNQ1725/PRO9925Dchil; DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; glycoprotein nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; Gpnmb; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; Hgfin; ipd; iris pigment dispersion; NMB; Osteoactivin; si:ch211-155k24.6; transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB; Unknown (protein for MGC:133733); UNQ1725/PRO9925;
Gene ID (Entrez) 10457
Gene Symbol GPNMB
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target GPNMB
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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