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  5. Invitrogen™ Monkey ANGPT2 ELISA Kit

Invitrogen™ Monkey ANGPT2 ELISA Kit

Catalog No. EP2RBX5
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5 x 96 Tests
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EP2RBX5 5 x 96 Tests
EP2RB 96 Tests
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Description

ELISA

Monkey ANGPT2 quantitates monkey ANGPT2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant monkey ANGPT2.

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

Specifications

Accession Number Agpt2; ANG2; Ang-2; angiopoietin 2; angiopoietin-2; angiopoietin-2 A; angiopoietin-2 B; ANGPT2; endothelial growth factor; Tie2-ligand
Assay Range 0.12 to 30 ng/mL
Assay Sensitivity 0.12 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Angiopoietin 2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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