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  5. Invitrogen™ Monkey ANGPT2 ELISA Kit

Invitrogen™ Monkey ANGPT2 ELISA Kit

Catalog No. EP2RB
Encompass
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5 x 96 Tests
96 Tests
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EP2RB 96 Tests
EP2RBX5 5 x 96 Tests
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Description

ELISA

Monkey ANGPT2 quantitates monkey ANGPT2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant monkey ANGPT2.

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

Specifications

Accession Number Agpt2; ANG2; Ang-2; angiopoietin 2; angiopoietin-2; angiopoietin-2 A; angiopoietin-2 B; ANGPT2; endothelial growth factor; Tie2-ligand
Assay Range 0.12 to 30 ng/mL
Assay Sensitivity 0.12 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Angiopoietin 2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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