Invitrogen™ X-Rhod-1, AM, cell permeant - Special Packaging

• Uses in many calcium signaling investigations, including measuring Ca²⁺ in cells and tissues
• Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells
• Fluorescence signal from these cells is generally measured using fluorescence microscopy
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺
• Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator
• Label (Ex/Em of Ca²⁺–bound form): X-rhod-1 (580/602nm)
• Fluorescence intensity increase upon binding Ca²⁺: >100 fold
• K_d for Ca²⁺ in the absence of Mg²⁺, in buffer: approximately 700nM

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling