Invitrogen™ Transferrin From Human Serum, Biotin-XX Conjugate

Transferrin is a monomeric serum glycoprotein (approx. 80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe³⁺ atoms via receptor-mediated endocytosis.

• Once iron-carrying transferrin proteins are inside endosomes, acidic environment favors dissociation of iron from transferrin-receptor complex
• Following release of iron, apotransferrin is recycled to plasma membrane, where it is released from its receptor to scavenge more iron
• Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish lysosomally directed and recycling endosomal pathways
• Experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy
• Label (Ex/Em): Alexa Fluor 594 (590/617)
• Amount: 15mg solid (contains 5mg of transferrin conjugate)
• Applications include: Image transferrin receptor dynamics using FRET; Observe receptor trafficking in live cells by confocal laser-scanning microscopy; Investigate events occurring during endosomal acidification; Measure transferrin receptor binding affinity in mammals and parasites

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Flow Cytometry, Flow Cytometry Endocytosis, Exocytosis and Phagocytosis Assays, Flow Cytometry of Cellular Processes, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Ionic Homeostasis and Signaling, pH Detection