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Invitrogen™ SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO

Catalog No. S11368
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S11368 250 μL
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Catalog No. S11368 Supplier Invitrogen™ Supplier No. S11368
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Description

Useful as an indicator of cell death

SYTOX Orange dye stains nucleic acids in cells with compromised membranes. This stain is much brighter than propidium iodide (P-1304). 5mM solution in DMSO.

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function, Cellular Imaging, Immunofluorescence (IF), Immunofluorescence Counterstaining, Mounting & Fade Prevention, Immunofluorescence Staining & Detection, Nucleus, Nucleoli & Nuclear Envelope, Organelle Tracing

Order Info

Shipping Condition: Room Temperature

Specifications

Color Orange
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Excitation Wavelength Range 547 nm
Dye Type Cell-Permeant
Format Tube(s)
For Use With (Equipment) Fluorescence Microscope
Quantity 250 μL
Volume (Metric) 250 μL
Detection Method Fluorescence
Emission 570 nm
Form Solution
Product Line SYTOX
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Nucleic Acid Stain
SubCellular Localization Nucleic Acids
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Frequently Asked Questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

For Research Use Only. Not for use in diagnostic procedures.