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Invitrogen™ SYTO™ Orange Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 80-85 - 50 μL each

Catalog No. S11360
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Catalog No. S11360 Supplier Invitrogen™ Supplier No. S11360
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Description

Includes cell-permeant, orange-fluorescent SYTO dyes

The SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit contains our collection of cell-permeant, orange-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 μL each of SYTO 80–85 dyes.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

Order Info

Shipping Condition: Room Temperature

Specifications

Color Orange
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Excitation Wavelength Range 531, 530, 541, 543, 567, 567 nm
Dye Type Cell-Permeant
For Use With (Equipment) Fluorescence Microscope
Quantity 1 kit
Volume (Metric) 50 μL
Detection Method Fluorescence
Description SYTO™ Orange Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 80-85, 50 μL each
Emission 545, 544, 560, 559, 582, 583 nm
Product Line SYTO
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Stains Dimer Sampler Kit
SubCellular Localization Nucleic Acids
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Frequently Asked Questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

For Research Use Only. Not for use in diagnostic procedures.