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Invitrogen™ SNARF™-4F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging

Catalog No. S23921
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Catalog No. S23921 Supplier Invitrogen™ Supplier No. S23921
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Description

Cell-permeant ratiometric pH indicator

  • pKa of approximately 6.4
  • Exhibits significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively
  • This pH dependence allows ratio of fluorescence intensities from dye at two emission wavelengths, typically 580nm and 640nm, to be used for quantitative determinations of pH

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, pH Detection

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product Type pH Indicator
Dye Type Fluorescent Dye-Based
Indicator pH Indicator
Product Line SNARF
Quantity 20 x 50 μg
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.