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Invitrogen™ SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells
Description
The SelectFX Nuclear Labeling Kit provides four spectrally distinct fluorescent dyes: blue-fluorescent DAPI, green-fluorescent SYTOX Green stain, red-fluorescent 7-aminoactinomycin D (7-AAD), and far red-fluorescent TO-PRO-3 dye. These dyes are ideal for use as counterstains in multicolor applications; simply select the stain that contrasts spectrally with other fluorescent probes applied to the sample. When used according to the protocol provided, the dyes in the SelectFX Nuclear Labeling Kit provide highly selective nuclear staining with little or no cytoplasmic labeling.
Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Immunofluorescence (IF), Immunofluorescence Counterstaining, Mounting & Fade Prevention, Immunofluorescence Staining & Detection, Nucleus, Nucleoli & Nuclear Envelope, Organelle Tracing
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Color | Green |
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Dye Type | Cell-Impermeant |
| For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
| Quantity | 1 kit |
| Detection Method | Fluorescence |
| Product Line | SELECTFX, SYTOX, TO-PRO |
| Shipping Condition | Room Temperature |
| Label Type | Fluorescent Dye |
| Product Type | Nuclear Labeling |
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Frequently Asked Questions (FAQs)
The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:
1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid.
4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).
SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.
Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.
SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.
For Research Use Only. Not for use in diagnostic procedures.