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Invitrogen™ SBFI, Tetraammonium Salt, cell impermeant 
Sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, or to measure Na+ efflux in cells
Supplier: Invitrogen™ S1262
Description
- Useful in combination with other fluorescent indicators used to correlate changes in intracellular Na+ with Ca2+ and Mg2+ concentrations, intracellular pH, and membrane potential
- Although selectivity of SBFI for Na+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na+ in presence of other monovalent cations
- The spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2
- Label (Ex/Em): SBFI (approximately 340,380/500nm)
- Lyophilized product may be dissolved in distilled water or aqueous buffer for use
- Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording
- Dissociation constant (Kd) of SBFI for Na+ is 3.8mm in the absence of K+, and 11.3mm in solutions with combined Na+ and K+ concentration of 135mm (which approximates physiological ionic strength). SBFI is approximately 18-fold more selective for Na+ than for K+
Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection
Order Info
Shipping Condition: Room temperature
Specifications
| Store at room temperature and protect from light. | |
| Fluorescence Microscope | |
| Sodium Indicator | |
| Room Temperature |
| Fluorescence | |
| SBFI Tetraammonium Salt | |
| 1 mg |
For Research Use Only. Not for use in diagnostic procedures.