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Invitrogen™ Rhod-5N, Tripotassium Salt, cell impermeant
Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipet, microinjection or pinocytotic cell-loading reagent
Supplier: Invitrogen™ R14207
Description
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.
- Fluorescence signal from these cells is generally measured using fluorescence microscopy
- Fluorescence intensity increase upon binding Ca2+: >100 fold
- Kd for Ca2+ in absence of Mg2+, in buffer: approximately 320μM
- Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
- In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
- Perturbations to calcium measurements caused by presence of these ions can be controlled using heavy metal-selective chelator TPEN
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling
Order Info
Shipping Condition: Room temperature
Spécifications
| Store in freezer -5°C to -30°C and protect from light. | |
| Fluorescence Microscope | |
| Fluorescent Dye-Based | |
| Room Temperature |
| Fluorescence | |
| Calcium Indicator | |
| 500 μg |
For Research Use Only. Not for use in diagnostic procedures.