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Invitrogen™ Qtracker™ 625 Cell Labeling Kit
Description
Includes
100μL Qtracker nanocrystals, Component A (2μM in 50mm borate buffer, pH 8.3) and 100μL Qtracker carrier, Component B (phosphate buffered saline, pH 7.2).
- Qtracker 625 label has Ex/Em 405-585nm/625nm
- Cytoplasmic delivery is not mediated by specific enzyme; therefore, no cell-type specificity has been observed
- Delivery is typically accomplished in less than 1 hour
- Qdot nanocrystals delivered are compatible with serum-sensitive cells
- Intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
- Observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes
- Distributed in vesicles in cytoplasm, and are inherited by daughter cells for at least six generations: Fluorescence from the Qtracker labels can be seen up to a week after delivery in some cell lines
- Long-term cellular retention makes kits suitable for studying cell motility, migration, differentiation, morphology, and many other cellular function studies
- Labels do not leak out of intact cells to be taken up by adjacent cells in population
- Can be easily monitored on variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
- Cytotoxicity of the materials use in Qtracker Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells: Labeling with Qtracker Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
- Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics
Cell Analysis, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, Chemotaxis and Cell Migration
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Contains 100 μl Qtracker™ nanocrystals, Component A (2 μM in 50 mM borate buffer, pH 8.3) and 100 μl Qtracker™ carrier,Component B (phosphate buffered saline, pH 7.2). Store at 2–6°C. Do not freeze. Stable for at least 6 months. |
| Quantity | 1 Kit |
| Product Line | Molecular Probes, QTRACKER |
| Reagent Type | Cell Labeling Reagents |
| Shipping Condition | Room Temperature |
| Product Type | Cell Labeling |
Frequently Asked Questions (FAQs)
Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.
Quantum dots can be excited by wavelengths of light ranging from 250 nm to 40 nm below the emission peak wavelength. For example, Qtracker 655 non-targeted quantum dots can be excited from 250 -615 nm. The wavelength in the product name refers to the emission peak, not the excitation peak. Since quantum dots have an exponential curve to their absorbance, the lower the wavelength at which they are excited, the more efficiently they will absorb, so you will want to use your lowest available laser line or excitation wavelength.
This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.
Please see this Web link (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html) to help you choose the right option for your application. Start by planning how long you want to track your cells, then consider the mechanism of binding. Calcein dyes are very uniform in label and are good for short-term cell migration, but may be rapidly effluxed from some cell types. Lipophilic cyanine dyes, such as DiI, DiO, and similar dyes label cell membranes, don’t disrupt function, and can last longer, but have the potential to cross to other cells if membranes fuse. They are also lost upon permeabilization. CellTracker dyes are better for longer-term labeling, as they possess a mildly reactive chloromethyl moiety that allows covalent binding to cellular components. CFDA SE also covalently binds to cellular components. With all the reagents, their retention within cells is dependent upon the rate of cell division and the inherent properties of the cell (active efflux, membrane and protein turnover rates, etc.) and reagents that allow for covalent attachment exhibit longer retention than those that do not.
The longest-lasting and brightest options are the Qtracker reagents, which are taken up through endocytosis. These are so bright individual quantum dots can be detected, and are also robust enough to survive not only fixation and permeabilization, but even the heat and solvents used in paraffin processing.
For Research Use Only. Not for use in diagnostic procedures.