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Invitrogen™ Newport Green™ DCF Diacetate, cell permeant

Catalog No. N7991
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N7991 1 mg
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Catalog No. N7991 Supplier Invitrogen™ Supplier No. N7991
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Description

Has moderate zinc-binding affinity (Kd for Zn2+ approximately 1μM) but is essentially insensitive to Ca2+

  • Kd for Ca2+ >100μM), making this valuable probe for detecting Zn2+ influx into neurons through voltage- or glutamate-gated channels
  • When used alongside dyes with dual Ca2+ / Zn2+ sensitivity such as fura-2 and mag-fura-2, provides confirmation that changes in Zn2+ levels, and not Ca2+ or Mg2+ are being detected
  • The response to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized
  • Label (Ex/Em): Newport Green DCF (approximately 505/535nm)
  • Kd (Zn2+) (in buffer): approximately 1μM
  • Lyophilized product may be dissolved in DMSO for use
  • Product is typically loaded into cells by adding dissolved indicator to medium containing cells

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Zinc and Heavy Metal Ion Detection

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Fluorescence Microscope
Product Type DCF Diacetate
Dye Type Zinc Indicator
Product Line Newport Green
Quantity 1 mg
Shipping Condition Room Temperature

For Research Use Only. Not for use in diagnostic procedures.