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Invitrogen™ Low Density Lipoprotein from Human Plasma, DiI complex (DiI LDL)

Catalog No. L3482
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200 μL
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L3482 200 μL
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Catalog No. L3482 Supplier Invitrogen™ Supplier No. L3482
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Description

Nonacetylated conjugate

Human low-density lipoprotein (LDL) is large protein complex (approx. 500,000 Da) that binds to a specific receptor on surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis. Experiments to study LDL are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively fluorescently labeled LDL can be injected into test animals, and distribution of the label can be analyzed after the specified time period.

  • Label (Ex/Em): Dil (554/571)
  • Acetylated: No
  • Amount: 200μL (1.0mg/mL
  • Nonacetylated LDL products are shipped within two weeks of their preparation
  • LDL containing unmodified apoprotein is used to study normal cholesterol delivery and internalization
  • Applications for labeled LDL complexes include: Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization; Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay); Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines

Cell Analysis, Cell Cycle, Cell Structure, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Lysosomes, Vacuoles

Order Info

Shipping Condition: Wet Ice

Specifications

Concentration 1 mg⁄ml
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Detection Method Fluorescence
Product Type Supplement
Dye Type Other Label(s) or Dye(s)
Form Liquid
Quantity 200 μL
Shipping Condition Wet Ice

Frequently Asked Questions (FAQs)

If the Low Density Lipoprotein (LDL) from Human Plasma was accidentally frozen, can I still use it?

We recommend storing Low Density Lipoprotein (LDL) from Human Plasma at 2-8 degrees C. We do not recommend using frozen product.

Can I use Low Density Lipoprotein (LDL) from Human Plasma on other species?

We have not tested this.

For Research Use Only. Not for use in diagnostic procedures.