Invitrogen™ Low Density Lipoprotein From Human Plasma, Acetylated, Alexa Fluor™ 488 Conjugate (Alexa Fluor™ 488 AcLDL)

Human low-density lipoprotein (LDL) is large protein complex (approx. 500,000 Da) that binds to a specific receptor on the surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis. Experiments to study LDL are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively the fluorescently labeled LDL can be injected into test animals, and the distribution of the label can be analyzed after the specified time period.

• Label (Ex/Em): Alexa Fluor488 (495/519)
• Acetylated: Yes
• Amount: 200μL (1.0mg/mL)
• LDL and AcLDL products from fresh human plasma approximately every two months and are available on continuous basis
• LDL containing an unmodified apoprotein is used to study normal cholesterol delivery and internalization: If the lysine residues of LDL apoprotein have been acetylated, the LDL complex no longer binds to the LDL receptor, but rather is taken up by endothelial and microglial cells that possess ;scavenger receptors specific for that modified form
• Some of many applications for labeled LDL complexes include: Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization, Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay), Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines

Cell Analysis, Cell Cycle, Cell Structure, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Lysosomes, Vacuoles