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Invitrogen™ LIVE/DEAD™ Sperm Viability Kit

Catalog No. L7011
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Catalog No. L7011 Supplier Invitrogen™ Supplier No. L7011

Description

Assay for viability and fertilizing potential of sperm

The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm by fluorescence microscopy or flow cytometry. Membrane-permeant SYBR™ 14 nucleic acid stain labels live sperm with green fluorescence, and membrane-impermeant propidium iodide labels the nucleic acids of membrane-compromised sperm with red fluorescence.

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes

Order Info

Shipping Condition: Room temperature

Specifications

Cell Type Eukaryotic Cells, Sperm Cells
Color Green, Red
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Description LIVE/DEAD™ Sperm Viability Kit
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Sperm Viability Kit
Dye Type Other Label(s) or Dye(s)
Emission 516/617
Format Tube(s), Slide(s)
Product Line LIVE/DEAD
Quantity 1 kit
Shipping Condition Room Temperature
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Frequently Asked Questions (FAQs)

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.
How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

For Research Use Only. Not for use in diagnostic procedures.