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Invitrogen™ LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

Catalog No. L7010
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L7010 1 kit
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Catalog No. L7010 Supplier Invitrogen™ Supplier No. LSL7010

Description

Red and green stains used

The LIVE/DEAD Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes

Order Info

Shipping Condition: Room temperature

Specifications

Cell Type Mammalian Cells, Eukaryotic Cells
Color Green, Red
Content And Storage Store in refrigerator at 2°C to 8°C and protect from light.
Description LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Cell-Mediated Cytotoxicity Kit
Dye Type Other Label(s) or Dye(s)
Emission 501/617
Excitation Wavelength Range 484, 536 nm
Format Tube(s), Slide(s)
Product Line LIVE/DEAD
Quantity 1 kit
Shipping Condition Room Temperature
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Frequently Asked Questions (FAQs)

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

For Research Use Only. Not for use in diagnostic procedures.