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Invitrogen™ HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging

Catalog No. H34477
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Catalog No. H34477 Supplier Invitrogen™ Supplier No. H34477
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Description

High affinity for neutral lipid droplets

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis.

Cell Analysis, Cellular Imaging, High-Content Screening (HCS)

Order Info

Shipping Condition: Room temperature

Specifications

Color Deep Red
Content And Storage Store in freezer -5°C to -30°C and protect from light.
For Use With (Equipment) High Content Instrument
Quantity 1 each
Detection Method Fluorescence
Product Line LipidTOX
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Lipid Stain
Sub Cellular Localization Cell Membranes, Lipids

Frequently Asked Questions (FAQs)

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains?

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.
Is the HCS LipidTOX Deep Red Neutral Lipid Stain (Cat. No. H34477) ready to use, or do I need to dilute it in 1X PBS or DMSO?

The HCS LipidTOX Deep Red Neutral Lipid Stain is provided as a solution in DMSO and needs to be diluted before use. The recommended dilution is 1:200 in 1X PBS or other buffer (e.g. HBSS, TBS, etc).
What buffer should I use with HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging (Cat. No. H3447)?

The ideal buffer with HCS LipidTOX Deep Red Neutral Lipid Stain is PBS at pH 7.4.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

For Research Use Only. Not for use in diagnostic procedures.