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Invitrogen™ Staphylococcus aureus (Wood strain without protein A) BioParticles™, Alexa Fluor™ 594 conjugate

Catalog No. S23372
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S23372 2 mg
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Catalog No. S23372 Supplier Invitrogen™ Supplier No. S23372
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Description

Fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities

Fluorescent products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

Unlike the fluorescence of fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.

  • Label (Ex/Em): Alexa Fluor 594 (approx. 590/617nm)
  • Particle: S. aureus (Wood strain, without protein A)
  • Provided as lyophilized powders
  • approximately 3 x 108 S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid
  • Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
  • Conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
  • Fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
  • Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Structure, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Receptors

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope
Product Type Bioparticle Conjugate
Dye Type Alexa Fluor™ 594
Form Lyophilized Powder
pH 3 to 10
Product Line Alexa Fluor, BioParticles
Quantity 2 mg
Shipping Condition Room Temperature
Species S. aureus
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Frequently Asked Questions (FAQs)

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.
What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

For Research Use Only. Not for use in diagnostic procedures.