Invitrogen™ Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 594 conjugate

Numéro de catalogue. E23370

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2 mg

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Numéro de catalogue.	Quantity
E23370	2 mg

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Numéro de catalogue. E23370 Fournisseur Invitrogen™ Code fournisseur E23370

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Description

The fluorescence of the dye conjugates is uniformly intense over the pH range from 3 to 10.

Conjugates covalently labeled with fluorophores (special care has been taken to remove free dye after conjugation).

Label (Ex/Em): Alexa Fluor594 (approx. 590/617nm)
Conjugates are provided as lyophilized powders
There are approximately 3 x 10⁸ E. coli or S. aureus particles per mg solid and approximately 2 x 10⁷ zymosan particles per mg solid
Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
Fluorescence of conjugates that are bound to surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
In addition to cellular applications, fluorescent conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Shipping Condition: Room Temperature

Content And Storage	Store in freezer (-5 to -30°C) and protect from light.
Detection Method	Fluorescence
For Use With (Equipment)	Fluorescence Microscope
Product Type	E.coli Conjugate
Dye Type	Alexa Fluor™ 594
Form	Lyophilized Powder
pH	3 to 10
Product Line	Alexa Fluor, BioParticles
Quantity	2 mg
Shipping Condition	Room Temperature
Species	E. coli
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For Research Use Only. Not for use in diagnostic procedures.