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Invitrogen™ HCS DNA Damage Kit

Catalog No. H10292
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H10292 1 kit
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Catalog No. H10292 Supplier Invitrogen™ Supplier No. H10292
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Description

Includes

Kit contains sufficient reagents for performing two plates in a 96-well plate format.

Detects DNA damage and cytotoxicity

The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage is detected using an antibody against phosphorylated H2AX (Ser139) which is induced in response to double-strand break (DSB) formation. To detect cytotoxicity, the Image-iT™ DEAD™ Green is an impermeant dye in healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.

Cell Analysis, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Dry ice

Specifications

Color Orange, Blue, Green
Content And Storage The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  • Store at ≤-20°C, desiccate and protect from light.
    		•
    Detection Method Fluorescence
    For Use With (Equipment) High Content Analysis Instrument
    Product Type DNA Damage kit
    Dye Type Alexa Fluor™ 555, Hoechst 33258
    Format 96-well plate
    Product Line Molecular Probes
    Quantity 1 kit
    Shipping Condition Dry Ice

    Frequently Asked Questions (FAQs)

    What are the fluorescence excitation/emission maxima for the dyes contained in the HCS DNA Damage Kit?

    Hoechst 33342: 350/461 nm, bound to DNA
    Image-IT Dead Green viability stain: 488/515 nm
    Alexa Fluor 555 goat anti-mouse IgG: 555/565 nm

    For Research Use Only. Not for use in diagnostic procedures.