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Invitrogen™ FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

Catalog No. T23360
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T23360 1 mg
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Catalog No. T23360 Supplier Invitrogen™ Supplier No. T23360
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For use with fluorescence microscopy

FM 5-95 is a slightly less lipophilic analog of FM 4-64 (T-3166, T-13320) with essentially identical spectroscopic properties.

Cell Analysis, Cell Tracing & Tracking, Cell Viability, Proliferation & Function, Endocytosis, Exocytosis & Phagocytosis, General Cell Tracing, Neuronal Tracing

Order Info

Shipping Condition: Room Temperature

Specifications

Color Red
Content And Storage Store at room temperature and protect from light.
For Use With (Equipment) Fluorescence Microscope
Quantity 1 mg
Detection Method Fluorescence
Product Line FM
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Dye
Sub Cellular Localization Plasma Membrane
I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

For Research Use Only. Not for use in diagnostic procedures.