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Invitrogen™ FM™ 2-10 (N-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)

Catalog No. T7508
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T7508 5 mg
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Catalog No. T7508 Supplier Invitrogen™ Supplier No. T7508
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Description

Excellent reagent for identifying actively firing neurons and investigating mechanisms of activity-dependent vesicle cycling

The FM 2-10 membrane probe is a water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained.

Cell Analysis, Cell Tracing & Tracking, Cell Viability, Proliferation & Function, Endocytosis, Exocytosis & Phagocytosis, General Cell Tracing, Neuronal Tracing

Order Info

Shipping Condition: Room Temperature

Specifications

Color Orange
Content And Storage Store at room temperature and protect from light.
For Use With (Equipment) Fluorescence Microscope
Quantity 5 mg
Detection Method Fluorescence
Product Line FM
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Membrane Stain
Sub Cellular Localization Plasma Membrane

Frequently Asked Questions (FAQs)

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

For Research Use Only. Not for use in diagnostic procedures.