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Invitrogen™ FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide)
Description
FM 1-43 is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing.
FM 1-43 membrane probe is also available as 1mg packaged into 10 vials, each containing 100μg (T-35356). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.
Cell Analysis, Cell Tracing & Tracking, Cell Viability, Proliferation & Function, Endocytosis, Exocytosis & Phagocytosis, General Cell Tracing, Neuronal Tracing
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Color | Red |
| Content And Storage | Store at room temperature and protect from light. |
| For Use With (Equipment) | Fluorescence Microscope |
| Quantity | 10 x 100μg |
| Detection Method | Fluorescence |
| Product Line | FM |
| Shipping Condition | Room Temperature |
| Label Type | Fluorescent Dye |
| Product Type | Dye |
| SubCellular Localization | Plasma Membrane |
Frequently Asked Questions (FAQs)
No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.
No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.
FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.
In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.
Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.
For Research Use Only. Not for use in diagnostic procedures.