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Invitrogen™ FluoZin™-3, AM, cell permeant

Catalog No. f24195
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100 μg
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F24195 100 μg
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Catalog No. F24195 Supplier Invitrogen™ Supplier No. F24195
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Description

Zn2+-selective indicator with a structure that resembles fluo-4

  • Exhibits high Zn2+-binding affinity that is unperturbed by Ca2+ concentrations up to at least 1μM
  • Exhibits >50-fold increase in fluorescence in response to saturating levels of Zn2+
  • Although imaging applications of FluoZin-3 are predominant, cell-based microplate assays and flow cytometry protocols have also been developed
  • Label (Ex/Em): FluoZin-3 (approximately 494/516nm)
  • Kd (Zn2+) (in buffer): approximately 15 nM
  • Lyophilized product may be dissolved in DMSO for use
  • Product is typically loaded into cells by adding the dissolved indicator to medium containing cells

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Drug Discovery and Development, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation, Zinc and Heavy Metal Ion Detection

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Fluorescence Microscope
Product Type Zinc Indicator
Dye Type Zinc Indicator
Product Line FluoZin
Quantity 100 μg
Shipping Condition Room Temperature
Molecular Weight (g/mol) 982.85 g/mol

Frequently Asked Questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.