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Invitrogen™ FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit, 200-2,000 assays

Catalog No. F2962
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Catalog No. F2962 Supplier Invitrogen™ Supplier No. F2962
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Description

Provide rotocols and reagents for analyzing cellular NA with blue-fluorescent Hoechst 33258 nucleic acid stain

  • Quantitation of cellular DNA is rapid, and all manipulations can be carried out in microplate wells
  • Cells are lysed by freezing them in distilled water, diluted dye solution is then added to lysed cells, and fluorescence is measured

Biochemical Toxicology Assays, Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Proliferation, Cell Viability, Proliferation and Function, Cellular Imaging, Cellular Toxicology Assays, Drug Discovery and Development, High-Content Screening (HCS), Target-Based ADME/Tox Assays

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in refrigerator (2–8°C) and protect from light.
Detection Method Fluorescence
Format 96-well Plate
Label or Dye Other Labels or Dyes
For Use With (Equipment) Microplate Reader
Product Line FluoReporter
Product Type dsDNA Quantitation Kit
Quantity 1 kit
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Can I use Hoechst dyes to do both DNA quantitation and cell number counting in plates?

Yes, this can be done in a 96-well plate-based format. Hoechst dyes will label all cells, live or dead (or even fixed). This requires the use of a standard curve of known cell numbers (up to 100,000 cells per well) and a standard curve using purified DNA, , up to 10 µg/mL. We offer a kit for this: FluoReporter Blue Fluorometric dsDNA Quantitation Kit, Cat. No. F2962.

For Research Use Only. Not for use in diagnostic procedures.