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Invitrogen™ Fluo-5F, Pentapotassium Salt, cell impermeant 
Analog of fluo-4 with lower Ca2+-binding affinity, making it suitable for detecting intracellular calcium levels in 1μM to 1mM range that would saturate esponse of fluo-3 and fluo-4
Supplier: Invitrogen™ F14221
Description
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+.
- Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet or microinjection or Influx™ pinocytotic cell-loading reagent
- Compatible with excitation at 488nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications
- Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
- BAPTA-based indicators bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
- Perturbations to calcium measurements caused by presence of the ions can be controlled using heavy metal-selective chelator
- Label (Ex/Em of Ca2+-bound form): Fluo-5N (494/516nm)
- Fluorescence intensity increase upon binding Ca2+: >100 fold
- Kd for Ca2+ in buffer: approximately 90μM
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling
Order Info
Shipping Condition: Room temperature
Specifications
| Store in freezer -5°C to -30°C and protect from light. | |
| Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager | |
| Fluorescent Dye-Based | |
| Room Temperature |
| Fluorescence | |
| Calcium Indicator | |
| 500 μg |
For Research Use Only. Not for use in diagnostic procedures.