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Invitrogen™ Fluo-5F, AM, cell permeant - Special Packaging

Numéro de catalogue. F14222
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10 x 50 μg
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Numéro de catalogue. Quantity
F14222 10 x 50 μg
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Numéro de catalogue. F14222 Fournisseur Invitrogen™ Code fournisseur F14222
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Description

Analog of fluo-4 with lower Ca2+-binding affinity, making it suitable for detecting intracellular calcium levels in 1μM to 1mM range that would saturate response of fluo-3 and fluo-4

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+.

  • Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet or microinjection or Influx™ pinocytotic cell-loading reagent
  • Compatible with excitation at 488nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • BAPTA-based indicators bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of the ions can be controlled using heavy metal-selective chelator
  • Label (Ex/Em of Ca2+-bound form): Fluo-5F (494/516nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in buffer: approximately 2.3μM

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

Spécifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product Type Calcium Indicator
Dye Type Fluorescent Dye-Based
Quantity 10 x 50 μg
Shipping Condition Room Temperature

For Research Use Only. Not for use in diagnostic procedures.