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Invitrogen™ EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

Catalog No. E6639
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E6639 1 Kit
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Catalog No. E6639 Supplier Invitrogen™ Supplier No. E6639
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Description

Detects metallo-, serine, acid, and sulfhydryl proteases

The EnzChek Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY TR-X dye has excitation and emission spectra similar to those of the Texas Red fluorophore.

  • Generalized mechanism allows for measurement of variety of proteases
  • Can be used to continuously measure protease kinetics
  • Excitation/emission similar to Texas Red™ dye (589/617nm)

Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence Intensity
For Use With (Application) Protease Assay
For Use With (Equipment) Fluorescence Microplate Reader
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Shipping Condition Room Temperature
Substrate Properties Protein-Based Substrate
Substrate Type Protease Substrate
Target Enzyme Proteases
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For Research Use Only. Not for use in diagnostic procedures.