Invitrogen™ EnzChek™ Phosphate Assay Kit 

Description

The EnzChek Phosphate Assay Kit provides a fast and sensitive spectrophotometric method for the quantification of inorganic phosphate in solution and for phosphate released during ATPase or GTPase enzymatic reactions. In the presence of inorganic phosphate, the 2-amino-6-mercapto-7-methylpurine riboside (MESG) substrate is converted by the purine nucleoside phosphorylase (PNP) enzyme to the ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine product. This enzymatic conversion of MESG results in a spectrophotometric shift in maximum absorbance from 330nm for the substrate to 360nm for the product. The reaction can be performed over a pH range of 6.5 to 8.5. The EnzChek phosphate reaction is sufficiently fast and quantitative for use in stopped-flow kinetic experiments.

• Sensitivity of 2 to 150μM phosphate
• Spectrophotometric microplate-based assay detects a change in absorbance maximum from 330nm to 360nm
• Continuous monitoring of phosphate generated by ATPase or GTPase activity

Anion Detection, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Ionic Homeostasis and Signaling, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

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Shipping Condition: Room temperature