Invitrogen™ EnzChek™ Phosphatase Assay Kit

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.

Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation