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Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

Catalog No. E33651
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Catalog No. E33651 Supplier Invitrogen™ Supplier No. E33651

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Measures amylase activity or screens amylase inhibitors in a high-throughput format

The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek™ kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

EnzChek™ Ultra Amylase Assay Kit Specifications:

  • Label (Ex/Em): BODIPY™ FL conjugate (∼502/512 nm)
  • Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
  • Sufficient reagents are supplied for 500 assays (using a 100 μL assay volume in a 96-well microplate assay format)

Find Fluorescent Substrates for Other Glycosidases

In addition to the EnzChek™ Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. Review Detecting Glycosidases—Section 10.2 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store at room temperature and protect from light.
Detection Method Fluorescence
For Use With (Application) Enzyme and Metabolic Activity
For Use With (Equipment) Microplate Reader
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Shipping Condition Room Temperature
Substrate Properties Molecular Substrate
Substrate Type Amylase Substrate
Target Enzyme Amylase
Excitation Wavelength 502 nm
Emission Wavelength 512 nm
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Frequently Asked Questions (FAQs)

What is the excitation/emission maxima of the digestion product from EnzChek Ultra Amylase Assay Kit?

The excitation/emission is 502/512nm.

For Research Use Only. Not for use in diagnostic procedures.