Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

The EnzChek Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

• Label (Ex/Em): BODIPY FL conjugate (approx. 502/512nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100μL assay volume in a 96-well microplate assay format)

Find Fluorescent Substrates for Other Glycosidases

In addition to the EnzChek Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more.

Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis