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Invitrogen™ ELF™ 97 Endogenous Phosphatase Detection Kit
Description
Upon hydrolysis the ELF 97 phosphatase substrate in the ELF 97 Endogenous Phosphatase Kit produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180nm that makes it easily distinguishable from endogenous fluorescence.
Cell Analysis, Cellular Imaging, Drug Discovery & Development, Enzyme & Protein Activity Assays, IHC Staining & Detection, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Staining & Detection, Immunohistochemistry (IHC), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation & Analysis, Target & Lead Identification & Validation
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Color | Yellow-Green |
| Content And Storage | Store in refrigerator 2°C to 8°C and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Phosphatase Assay |
| For Use With (Equipment) | Fluorescence Microscope |
| Product Type | Phosphatase Assay |
| Dye Type | ELF 97 |
| Emission | 345/530 |
| Form | Liquid |
| Product Line | ELF |
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Frequently Asked Questions (FAQs)
ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).
No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.
For Research Use Only. Not for use in diagnostic procedures.