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Invitrogen™ Di-8-ANEPPS
Description
Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play a central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating. Potentiometric probes are important tools for studying these processes.
- Fast-response probes that operate by means of change in their electronic structure, and consequently their fluorescence properties, in response to change in surrounding electric field
- Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains
- However, magnitude of their potential-dependent fluorescence change is often small; fast-response probes show 2-10% fluorescence change per 100 mV
- Display potential-dependent shift in their excitation spectra, thus permitting quantitation of membrane potential using excitation ratio measurements
- Zwitterionic molecule is less susceptible to internalization, permitting extended observation
- Ex/Em maxima bound to model phospholipid membranes are approximately 465/635nm (but spectral properties are highly dependent on environment)
- Nonfluorescent until bound to membranes
- Soluble in ethanol, DMSO, and DMF
- Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using retrograde labeling
- Plasma membrane of cell typically has transmembrane potential of approximately -70mV (negative inside) as consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes
- Potentiometric probes offer iindirect method of detecting translocation of these ions
Cell Analysis, Cell Metabolism, Cell Structure, Cell Viability, Proliferation and Function, Membranes (General) and Lipids
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Color | Orange |
| Content And Storage | Store at room temperature and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Fluorescence Microscope |
| Product Type | ANEP Dye |
| Quantity | 5 mg |
| Shipping Condition | Room Temperature |
| Sub Cellular Localization | Cell Membranes & Lipids |
Frequently Asked Questions (FAQs)
Di-8-ANEPPS dye (Cat. No. D3167) is essentially nonfluorescent until bound to membranes. The excitation/emission maxima for Di-8-ANEPPS when bound to model phospholipid membranes are ~465/635 nm but spectral properties are highly dependent on the environment.
If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).
Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.
A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).
For Research Use Only. Not for use in diagnostic procedures.