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Invitrogen™ Click-IT™ Biotin Protein Analysis Detection Kit DFS Item

Identify and characterize glycoproteins by Western blot

Supplier:  Invitrogen™ C33372

Catalog No. C33372


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Description

Description

The Click-iT Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT metabolic labeling reagent or the Click-iT Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. With this technique, detection sensitivity is in the low femptomole range and biotin-labeled samples can be detected before or after probing the Western with a primary antibody.

  • Less hazardous

Cell Analysis, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis, Western Blotting

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Less hazardous
1 Kit
Biotin-based, Fluorescence
Click-iT
Proteins
Biotin Protein Analysis Detection Kit
Store in freezer -5°C to -30°C.
Room Temperature
Biotin
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For Research Use Only. Not for use in diagnostic procedures.