missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein Labeling Reagents
  6. C10102

Invitrogen™ Click-IT™ AHA (L-Azidohomoalanine) DFS Item
GREENER_CHOICE

Non-radioactive technique

Supplier:  Invitrogen™ LSC10102

Catalog No. C10102


Only null left
Explore available promotions

Description

Description

Click-iT AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or 'click' reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT Protein Analysis Detection Kits containing either TAMRA, Dapoxyl™, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI MS analysis or Multiplexed Proteomics™ reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein.

  • Less hazardous

Cell Analysis, Click Chemistries, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry, Protein and Antibody Labeling, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Greener Choice Claims
  • Environmental benefits include:
    • Source Reduction
Specifications

Specifications

Less hazardous
5 mg
Biotin-based, Fluorescence
Room Temperature
Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)
AHA
Store in freezer -5°C to -30°C.
Metabolic Labeling
Click-iT™, Molecular Probes™
Proteins
Product Suggestions

Product Suggestions

Videos
SDS
Documents Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.