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Invitrogen™ CellLight™ Null (control), BacMam 2.0

Catalog No. C10615
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C10615 1 mL
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Catalog No. C10615 Supplier Invitrogen™ Supplier No. C10615
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Description

Intended as negative transduction control for CellLight fluorescent protein live cell staining reagents

CellLight Null Control contains no mammalian promoter and no transgene. CellLight reagents combine the utility and selectivity of fluorescent proteins with the transduction efficiency of BacMam technology, enabling unambiguous staining of organelles, cellular structures, and processes in live mammalian cells. They are provided in a ready-to-use format—simply add, incubate, and image—with highly efficient expression in cell lines, primary cells, stem cells, and neurons.Other CellLight reagents are fusion constructs of signal peptides or cell structure proteins with premiere emGFP, TagRFP or CFP for accurate and specific targeting to sub-cellular compartments and structures. A variety of targets, including cytoskeleton, mitochondria, and secretory compartments, are available for convenient multiplexing, co-localization studies, and imaging of dynamic cellular processes where high spatial and temporal resolution is required. The CellLight reagents tolerate fixation with formaldehyde and are therefore compatible with fixed-cell analysis.

  • Highly Efficient: >90% transduction of wide range of mammalian cell lines, including primary cells, stem cells, and neurons
  • Fast and Convenient: Simply add CellLight Null Control reagent to cells, incubate overnight, and evaluate how well cells tolerate BacMam transduction
  • Safe: CellLight reagents are non-replicating in mammalian cells, lack of observable cytopathic effect, and are suitable for biosafety level (BSL) 1 handling

Cell Analysis, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function

Order Info

Shipping Condition: Wet Ice

Specifications

Color Colorless
Content And Storage Store at 2°C to 6°C, protected from light. Do Not Freeze.
Dye Type Unconjugated
For Use With (Equipment) Confocal Microscope, Fluorescence Microscope
Quantity 1 mL
Detection Method Fluorescence
Emission Visible
Form Liquid
Product Line CellLight
Shipping Condition Wet Ice
Label Type Unlabeled
Product Type Null Control
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Frequently Asked Questions (FAQs)

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).
Is there any way to preserve the CellLights labeling beyond 5 days?

Cells transduced with the CellLights reagents can be stored frozen for several months after transduction, without loss of expression.
Are the CellLights products toxic to cells?

If the viral particles are used at the level we recommend, they are very well tolerated by cells.
For how long will the CellLights products label my cells?

The BacMam 2.0 CellLights typically express for 5 days after transduction.
What cell types can the CellLights products be used with?

The first generation BacMam reagents were shown to efficiently transduce over 90 cell types, including stable cell lines and primary cells. With BacMam 2.0, it is now possible to also efficiently transduce primary neurons and stem cells.
What safety precautions need to be taken to work with the BacMam reagent?

Our BacMam constructs do not replicate in mammalian cells, making them Biosafety Level I (the lowest biosafety level). They can be handled with the standard precautions used for any cell-based reagents.
Do CellLight BacMam 2.0 reagents work on all cell types?

BacMam delivery technology works with over 90 mammalian cell types. However, cells with hematopoietic origin do not show effective transduction with CellLight BacMam 2.0 reagents.

I keep getting low transduction efficiency when using CellLight labeling reagents on my neurons. What can I do to improve the efficiency?

Neurons are more difficult to transduce than many other cells. The main way to improve transduction is to label with a higher number of particles per cell. For primary neurons, it can also help to transduce them at the time of plating rather than on established cultures. There can also be a slower onset of expression in neurons and peak expression often occurs on day 2-3 rather than 16 hours after transduction.

For Research Use Only. Not for use in diagnostic procedures.