Invitrogen™ Calcium Green™-1, AM, cell permeant 

Description

• They have uses in many calcium signaling investigations, including measuring intracellular Ca²⁺, following Ca²⁺ influx and release, and multiphoton excitation imaging of Ca²⁺ in living tissues
• Cells may be loaded with the AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
• Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
• Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem

• Label (Ex/Em): Calcium Green; -1 (506/531nm)
• Fluorescence intensity increase upon binding Ca²⁺ : approximately 14 fold

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

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