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Invitrogen™ Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA
Description
Determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using a fluorometer or quantitative imaging system
- Kd of indicator can be calculated from plot generated by scanning excitation or emission of indicator in presence of eleven different Ca2+ concentrations
- Calcium Calibration Kit #1 contains 50mL of 10 mm K2EGTA and 50mL of 10 mm CaEGTA
- Both solutions contain 100 mm KCl and 30 mm MOPS, pH 7.2 and are prepared in deionized water
- These stock solutions can be blended to make buffers having free Ca2+ ranging from 0μM to 39μM
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in refrigerator at 2°C to 8°C |
| Detection Method | Fluorescence |
| For Use With (Application) | Calcium Calibration |
| For Use With (Equipment) | Fluorescence Microscope, Fluorometer |
| Product Type | Calcium Calibration Buffer Kit |
| Dye Type | Other Label(s) or Dye(s) |
| Format | Tube(s), Slide(s) |
| Quantity | 1 Kit |
| Shipping Condition | Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.