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Invitrogen™ ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

Catalog No. E34251
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100 μg
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E34251 100 μg
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Catalog No. E34251 Supplier Invitrogen™ Supplier No. E34251

Description

For live-cell imaging

ER-Tracker Green dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Endoplasmic Reticulum (ER), Immunofluorescence (IF), Immunofluorescence Staining & Detection, Organelle Tracing

Order Info

Shipping Condition: Room Temperature

Specifications

Color Green
Content And Storage Store in freezer -5°C to -30°C and protect from light.
For Use With (Equipment) Fluorescence Microscope
Quantity 100 μg
Detection Method Fluorescence
Product Line BODIPY, ER-Tracker
Shipping Condition Room Temperature
Label Type BODIPY Dyes
Product Type Dye
SubCellular Localization Endoplasmic Reticulum

Frequently Asked Questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.