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Invitrogen™ BCEF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester) Solution 
For measuring changes in the cytosolic pH of most cells
Supplier: Invitrogen™ B3051
Description
- BCECF, AM is cell-permeant, dual-excitation ratiometric pH indicator with a pKa of approximately 6.98
- Intracellular pH measurements with BCECF are made by determining pH-dependent ratio of emission intensity (detected at 535nm) when dye is excited at approximately 490nm versus emission intensity when excited at its isobestic point of approximately 440nm
Cell Adhesion, Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation, pH Detection
Order Info
Shipping Condition: Room temperature
Specifications
| 1 mg⁄ml | |
| Fluorescence | |
| BCEF AM pH indicator | |
| 1 mL |
| Store in freezer -5°C to -30°C. | |
| Fluorescence Microscope, Flow Cytometer, Microplate Reader | |
| pH Indicator | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.