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  6. Invitrogen™ BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)

Invitrogen™ BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)

Catalog No. b1150
Encompass
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20 x 50 μg
1 mg
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B1150 1 mg
B1170 20 x 50 μg
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Description

For measuring changes in the cytosolic pH of most cells

  • With pKa of approximately 6.98, cell-permeant, dual-excitation ratiometric pH indicator, AM is ideal for measuring changes in cytosolic pH of most cells
  • Intracellular pH measurements with BCECF are made by determining pH-dependent ratio of emission intensity (detected at 535nm) when dye is excited at approximately 490nm versus emission intensity when excited at its isobestic point of approximately 440nm

Cell Adhesion, Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation, pH Detection

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C.
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Product Type pH indicator
Dye Type pH Indicator
Quantity 1 mg
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.