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Invitrogen™ Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

Catalog No. A22182
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400 Assays
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A22182 400 Assays
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Catalog No. A22182 Supplier Invitrogen™ Supplier No. A22182
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Description

Suitable for cell viability, proliferation and Function, cellular imaging, and enzyme and protein activity assays

  • Provides sensitive and simple fluorometric method for detecting as little as 200nM xanthine or hypoxanthine or as little as 0.1mU/mL of xanthine oxidase activity in a purified system with a 100μL assay volume

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Xanthine/Xanthine Oxidase Assay
For Use With (Equipment) Microplate Reader, Spectrophotometer, Fluorometer
Product Type Amplex Red Assay Kit
Dye Type Other Label(s) or Dye(s)
Format Tube(s), 96-well plate
Product Line Amplex
Quantity 400 Assays
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.
Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

For Research Use Only. Not for use in diagnostic procedures.